SUPPOSE THAT YOU WANTED TO USE BACTERIA TO EXPRESS THE

SUPPOSE THAT YOU WANTED TO USE BACTERIA TO EXPRESS THE

SUPPOSE THAT YOU WANTED TO USE BACTERIA TO EXPRESS THE PRODUCT OF A EUKARYOTIC GENE. GIVEN THE DIFFERENCES BETWEEN BACTERIAL AND EUKARYOTIC MRNA PRODUCTION COVERED IN TOPIC 9 WHY WOULD YOU USE A CDNA CLONE INSTEAD OF A GENOMIC DNA CLONE?
 
 
WHAT WOULD A BACTERIAL EXPRESSION VECTOR NEED TO HAVE BESIDES THE SITE TO INSERT THE CDNA?
Suppose that you wanted to use bacteria to express the product of a eukaryotic gene. Given the differences between bacterial and eukaryotic mRNA production covered in Topic 9, why would you use a cDNA clone instead of a genomic DNA clone? What would a bacterial expression vector need to have besides the site to insert the cDNA?

 
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